DNMT3B Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using DNMT3B mAb against human DNMT3B (AA: 1-150) recombinant protein. (Expected MW is 42.6 kDa)
Figure 3:Western blot analysis using DNMT3B mAb against HEK293 (1) and DNMT3B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of K562 cells using DNMT3B mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using DNMT3B mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DNMT3B mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	Q9UBC3
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7E5E9C1
Isotype	IgG1
Calculated MW	95751 Da
Description	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Immunogen	Purified recombinant fragment of human DNMT3B (AA: 1-150) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, 2.1.1.37, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DNMT3B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.