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HIST2H3C(27Ac) Antibody

Purified Mouse Monoclonal Antibody

     
  • 10 - HIST2H3C(27Ac) Antibody AO2159a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • 1 - HIST2H3C(27Ac) Antibody AO2159a

    Figure 2:Western blot analysis using HIST2H3C(27Ac) mouse mAb against Hela (1), Lncap (2), Jurkat (3), and Jurkat (4) cell lysate.

  • 4 - HIST2H3C(27Ac) Antibody AO2159a

    Figure 3:Flow cytometric analysis of Raji cells using HIST2H3C(27Ac) mouse mAb (green) and negative control (red).

  • 2 - HIST2H3C(27Ac) Antibody AO2159a

    Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using HIST2H3C(27Ac) mouse mAb with DAB staining.

  • 2 - HIST2H3C(27Ac) Antibody AO2159a

    Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HIST2H3C(27Ac) mouse mAb with DAB staining.

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);

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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC, E
Primary Accession Q71DI3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6E7A9
Isotype IgG1
Calculated MW 15388 Da
Description Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
Immunogen Synthesized peptide of human HIST2H3C (AA: ATKAARK(Ac)SAPATGGV).
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 126961;333932;653604
Other Names Histone H3.2, H3-clustered histone 13 {ECO:0000312|HGNC:HGNC:25311}, H3-clustered histone 14 {ECO:0000312|HGNC:HGNC:20503}, H3-clustered histone 15 {ECO:0000312|HGNC:HGNC:20505}, Histone H3/m, Histone H3/o, H3C15 (HGNC:20505)
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST2H3C(27Ac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H3C15 (HGNC:20505)
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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