FOXP3 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 2:Western blot analysis using FOXP3 mAb against human FOXP3 (AA: 297-431) recombinant protein. (Expected MW is 42 kDa)
Figure 3:Western blot analysis using FOXP3 mAb against HEK293 (1) and FOXP3 (AA: 297-431)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of HeLa cells using FOXP3 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FOXP3 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using FOXP3 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using FOXP3 mouse mAb with DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	Q9BZS1
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4F12A2
Isotype	IgG1
Calculated MW	47244 Da
Description	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen	Purified recombinant fragment of human FOXP3 (AA: 297-431) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	50943
Other Names	Forkhead box protein P3, Scurfin, FOXP3, IPEX
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	FOXP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	FOXP3
Synonyms	IPEX
Function	Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479, PubMed:24835996, PubMed:30513302, PubMed:32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed:23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00089, ECO:0000269\|PubMed:17360565, ECO:0000269\|PubMed:18354202, ECO:0000269\|PubMed:22678915, ECO:0000269\|PubMed:23396208, ECO:0000269\|PubMed:23973222, ECO:0000269\|PubMed:23973223, ECO:0000269\|PubMed:32644293}. Cytoplasm Note=Predominantly expressed in the cytoplasm in activated conventional T-cells whereas predominantly expressed in the nucleus in regulatory T- cells (Treg). The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells (By similarity). {ECO:0000250\|UniProtKB:Q99JB6, ECO:0000269\|PubMed:22678915}