TSHB Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using TSHB mAb against human TSHB (AA: 20-139) recombinant protein. (Expected MW is 16 kDa)
Figure 3:Flow cytometric analysis of Hela cells using TSHB mouse mAb (green) and negative control (red).
Figure 4:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TSHB mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded pancreas tissues using TSHB mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P01222
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1D12G1
Isotype	IgG2b
Calculated MW	15639 Da
Description	The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants.
Immunogen	Purified recombinant fragment of human TSHB (AA: 20-139) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, TSH-B, TSH-beta, Thyrotropin beta chain, Thyrotropin alfa, TSHB
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TSHB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.