ULK2 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ULK2 mAb against human ULK2 (AA: 1-155) recombinant protein. (Expected MW is 43.4 kDa)
Figure 3:Western blot analysis using ULK2 mAb against HEK293 (1) and ULK2 (AA: 1-155)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ULK2 mouse mAb against NIH/3T3 (1), HepG2 (2), SK-Hep-1 (3), SK-OV-3 (4), C6 (5), PC-12 (6), and MCF-7 (7) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using ULK2 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q8IYT8
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Clone Names	2H4B2
Isotype	IgG1
Calculated MW	112694 Da
Description	This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified.
Immunogen	Purified recombinant fragment of human ULK2 (AA: 1-155) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	9706
Other Names	Serine/threonine-protein kinase ULK2, 2.7.11.1, Unc-51-like kinase 2, ULK2, KIAA0623
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ULK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ULK2
Synonyms	KIAA0623
Function	Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.
Cellular Location	Cytoplasmic vesicle membrane; Peripheral membrane protein. Note=Localizes to pre-autophagosomal membrane

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.