GFPT1 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GFPT1 mAb against human GFPT1 (AA: 536-681) recombinant protein. (Expected MW is 42.3 kDa)
Figure 3:Western blot analysis using GFPT1 mAb against HEK293 (1) and GFPT1 (AA: 536-681)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using GFPT1 mouse mAb against Hela (1), HepG2 (2), HEK293 (3), BEL-7402 (4), SMMC-7721 (5), SK-MES-1 (6), C6 (7), and COS7 (8) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using GFPT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of Hela cells using GFPT1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, ICC, E
Primary Accession	Q06210
Reactivity	Human, Rat, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	1F1B9
Isotype	IgG1
Calculated MW	78806 Da
Description	This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
Immunogen	Purified recombinant fragment of human GFPT1 (AA: 536-681) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2673
Other Names	Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1, 2.6.1.16, D-fructose-6-phosphate amidotransferase 1, Glutamine:fructose-6-phosphate amidotransferase 1, GFAT 1, GFAT1, Hexosephosphate aminotransferase 1, GFPT1, GFAT, GFPT
Dilution	WB~~1/500 - 1/2000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GFPT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GFPT1
Synonyms	GFAT, GFPT
Function	Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes BMAL1 and CRY1 (By similarity). Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and its effects on hyaluronan synthesis that occur during tissue remodeling (PubMed:26887390).
Tissue Location	Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.