KDM6A Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using KDM6A mAb against human KDM6A (AA: 1252-1401) recombinant protein. (Expected MW is 43.4 kDa)
Figure 3:Western blot analysis using KDM6A mAb against HEK293 (1) and KDM6A (AA: 1252-1401)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using KDM6A mouse mAb against HepG2 (1) and C6 (2) cell lysate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	O15550
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Clone Names	6E2D6
Isotype	IgG2a
Calculated MW	154177 Da
Description	This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene.
Immunogen	Purified recombinant fragment of human KDM6A (AA: 1252-1401) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	7403
Other Names	Lysine-specific demethylase 6A, 1.14.11.-, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, KDM6A, UTX
Dilution	WB~~1/500 - 1/2000 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	KDM6A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	KDM6A
Synonyms	UTX
Function	Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17713478, PubMed:17761849, PubMed:17851529). Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed:17713478, PubMed:17761849, PubMed:17851529). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed:17761849). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).
Cellular Location	Nucleus.