ALDH6A1 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ALDH6A1 mAb against human ALDH6A1 (AA: 1-195) recombinant protein. (Expected MW is 47.2 kDa)
Figure 3:Western blot analysis using ALDH6A1 mAb against HEK293 (1) and ALDH6A1 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ALDH6A1 mouse mAb against Jurkat (1), HEK293 (2), Hela (3), MCF-7 (4), and LNcap (5) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 6:Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 7:Flow cytometric analysis of Hela cells using ALDH6A1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, ICC, E
Primary Accession	Q02252
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6H9B8
Isotype	IgG1
Calculated MW	57840 Da
Description	This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants.
Immunogen	Purified recombinant fragment of human ALDH6A1 (AA: 1-195) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	4329
Other Names	Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MMSDH, Malonate-semialdehyde dehydrogenase [acylating], 1.2.1.18, 1.2.1.27, Aldehyde dehydrogenase family 6 member A1, ALDH6A1, MMSDH
Dilution	WB~~1/500 - 1/2000 IHC~~1:100~500 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ALDH6A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ALDH6A1 (HGNC:7179)
Function	Malonate and methylmalonate semialdehyde dehydrogenase involved in the catabolism of valine, thymine, and compounds catabolized by way of beta-alanine, including uracil and cytidine.
Cellular Location	Mitochondrion.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.