CD33 Antibody

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);
Figure 1: Western blot analysis using DNAL4 mAb against human DNAL4 recombinant protein. (Expected MW is 44.7 kDa)
Figure 2: Western blot analysis using DNAL4 mAb against HEK293 (1) and DNAL4 (AA: 1-105)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DNAL4 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DNAL4 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, E
Primary Accession	P20138
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2C6B7
Isotype	IgG1
Calculated MW	39825 Da
Description	DNAL4 is a component of the dynein motor complex
Immunogen	Purified recombinant fragment of human DNAL4 (AA: 1-105) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3
Dilution	WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1:10~50 E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.