Mouse Monoclonal Antibody to DNTT

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using DNTT mAb against human DNTT (AA: 52-192) recombinant protein. (Expected MW is 42 kDa)
Western blot analysis using DNTT mAb against HEK293 (1) and DNTT (AA: 52-192)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using DNTT mouse mAb against Raji (1), A549 (2), Hela (3), and PC-12 (4) cell lysate.
Flow cytometric analysis of *** cells using Hela mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	P04053
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Clone Names	4B10A6
Isotype	Mouse IgG1
Calculated MW	58536 Da
Description	This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. ;
Immunogen	Purified recombinant fragment of human DNTT (AA: 52-192) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide
Application Note	ELISA: 1/10000; WB: 1/500 - 1/2000; FCM: 1/200 - 1/400

Additional Information
Gene ID	1791
Other Names	TDT
Dilution	WB~~1:1000 FC~~1:10~50 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Mouse Monoclonal Antibody to DNTT is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DNTT
Synonyms	TDT {ECO:0000303\|PubMed:11473582}
Function	Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Cellular Location	Nucleus.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.