Mouse Monoclonal Antibody to NS1

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using NS1 mAb against human Parvovirus NS1 (AA: 1-216) recombinant protein. (Expected MW is 33.4 kDa)
Western blot analysis using NS1 mAb against HEK293 (1) and NS1 (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric analysis of Hela cells using NS1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8E6H12
Isotype	Mouse IgG1
Calculated MW	7 KDa
Immunogen	Purified recombinant fragment of human Parvovirus NS1 (AA: 1-216) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide
Application Note	ELISA: 1/10000; WB: 1/500 - 1/2000; FCM: 1/200 - 1/400

Additional Information
Other Names	Human parvovirus B19 isolate Vn115 NS1
Dilution	WB~~1:1000 FC~~1:10~50 E~~N/A
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Mouse Monoclonal Antibody to NS1 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.