TFAP2A

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using TFAP2A mAb against human TFAP2A (AA: 105-211) recombinant protein. (Expected MW is 37.5 kDa)
Figure 3:Western blot analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 105-211)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using TFAP2A mouse mAb against Hela (1), PANC-1 (2), HEK293 (3), and MCF-7 (4) cell lysate.
Figure 7:Flow cytometric analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).
Figure 5:Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 6:Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P05549
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2H9B5
Isotype	Mouse IgG1
Calculated MW	48062 Da
Immunogen	Purified recombinant fragment of human TFAP2A (AA: 105-211) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	7020
Other Names	AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TFAP2A is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TFAP2A
Synonyms	AP2TF, TFAP2
Function	Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Cellular Location	Nucleus.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.