NAA10

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using NAA10 mAb against human NAA10 (AA: 111-235) recombinant protein. (Expected MW is 47.2 kDa)
Figure 3:Western blot analysis using NAA10 mAb against HEK293 (1) and NAA10 (AA: 111-235)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using NAA10 mouse mAb against COS7 (1), HEK293 (2), HL-60 (3), MCF-7 (4), Hela (5), NIH/3T3 (6), and C2C12 (7) cell lysate.
Figure 6:Flow cytometric analysis of SMMC-7721 cells using NAA10 mouse mAb (green) and negative control (red).
Figure 5:Immunofluorescence analysis of Hela cells using NAA10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P41227
Reactivity	Human, Mouse, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	3G3B9
Isotype	Mouse IgG1
Calculated MW	26459 Da
Immunogen	Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	8260
Other Names	TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	NAA10 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NAA10
Synonyms	ARD1, ARD1A, TE2
Function	Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19420222, PubMed:19826488, PubMed:20145209, PubMed:20154145, PubMed:25489052, PubMed:27708256, PubMed:29754825, PubMed:32042062). Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256). Acetylates HIST1H4A (PubMed:29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821).
Cellular Location	Cytoplasm. Nucleus. Note=Also present in the free cytosolic and cytoskeleton-bound polysomes.
Tissue Location	Ubiquitous..