NAA10

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using NAA10 mAb against human NAA10 (AA: 111-235) recombinant protein. (Expected MW is 47.2 kDa)
Figure 3:Western blot analysis using NAA10 mAb against HEK293 (1) and NAA10 (AA: 111-235)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using NAA10 mouse mAb against COS7 (1), HEK293 (2), HL-60 (3), MCF-7 (4), Hela (5), NIH/3T3 (6), and C2C12 (7) cell lysate.
Figure 6:Flow cytometric analysis of SMMC-7721 cells using NAA10 mouse mAb (green) and negative control (red).
Figure 5:Immunofluorescence analysis of Hela cells using NAA10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P41227
Reactivity	Human, Mouse, Monkey
Host	Mouse
Clonality	Monoclonal
Clone Names	3G3B9
Isotype	Mouse IgG1
Calculated MW	26459 Da
Immunogen	Purified recombinant fragment of human NAA10 (AA: 111-235) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	8260
Other Names	TE2; ARD1; NATD; ARD1A; ARD1P; OGDNS; DXS707; MCOPS1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	NAA10 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	NAA10
Synonyms	ARD1, ARD1A, TE2
Function	Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19420222, PubMed:19826488, PubMed:20145209, PubMed:20154145, PubMed:25489052, PubMed:27708256, PubMed:29754825, PubMed:32042062). Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256). Acetylates HIST1H4A (PubMed:29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821).
Cellular Location	Cytoplasm. Nucleus. Note=Also present in the free cytosolic and cytoskeleton-bound polysomes.
Tissue Location	Ubiquitous..