ASF1B

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ASF1B mAb against human ASF1B (AA: 1-202) recombinant protein. (Expected MW is 48.4 kDa)
Figure 3:Western blot analysis using ASF1B mAb against HEK293 (1) and ASF1B (AA: 1-202)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ASF1B mouse mAb against A549 (1) cell lysate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q9NVP2
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1A6G4
Isotype	Mouse IgG1
Calculated MW	22434 Da
Immunogen	Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	55723
Other Names	CIA-II
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ASF1B is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ASF1B {ECO:0000303\|PubMed:11470414, ECO:0000312\|HGNC:HGNC:20996}
Function	Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251, PubMed:21454524, PubMed:26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed:20953179, PubMed:21454524, PubMed:26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Required for gonad development (PubMed:12842904).
Cellular Location	Nucleus. Cytoplasm, cytosol
Tissue Location	Highly expressed in testis and at lower levels in colon, small intestine and thymus.