TFAP2C

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using TFAP2C mAb against human TFAP2C (AA: 341-450) recombinant protein. (Expected MW is 38.2 kDa)
Figure 3:Western blot analysis using TFAP2C mAb against HEK293 (1) and TFAP2C (AA: 341-450)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using TFAP2C mouse mAb against SK-Br-3 (1) cell lysate.
Figure 5:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using TFAP2C mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q92754
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3A11A5
Isotype	Mouse IgG1
Calculated MW	49177 Da
Immunogen	Purified recombinant fragment of human TFAP2C (AA: 341-450) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	7022
Other Names	ERF1; TFAP2G; hAP-2g; AP2-GAMMA
Dilution	WB~~ 1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TFAP2C is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TFAP2C
Function	Sequence-specific DNA-binding transcription factor that interacts with cellular enhancer elements to regulate transcription of selected genes, and which plays a key role in early embryonic development (PubMed:11694877, PubMed:24413532). AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions (PubMed:11694877, PubMed:24413532). TFAP2C plays a key role in early embryonic development by regulating both inner cell mass (ICM) and trophectoderm differentiation (By similarity). At the 8-cell stage, during morula development, controls expression of cell-polarity genes (By similarity). Upon trophoblast commitment, binds to late trophectoderm genes in blastocysts together with CDX2, and later to extra-embryonic ectoderm genes together with SOX2 (By similarity). Binds to both closed and open chromatin with other transcription factors (By similarity). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532).
Cellular Location	Nucleus.