RIPK4

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using RIPK4 mAb against human RIPK4 (AA: 675-784) recombinant protein. (Expected MW is 37.7 kDa)
Figure 3:Western blot analysis using RIPK4 mAb against HEK293 (1) and RIPK4 (AA: 675-784)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of SK-N-SH cells using RIPK4 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q9H4D1
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1F7D9
Isotype	Mouse IgG1
Calculated MW	86408 Da
Immunogen	Purified recombinant fragment of human RIPK4 (AA: 675-784) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	54101
Other Names	DIK; PKK; PPS2; RIP4; ANKK2; NKRD3; ANKRD3
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RIPK4 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	dik {ECO:0000313\|EMBL:CAC04247.1}
Function	Serine/threonine protein kinase. Required for embryonic skin development and correct skin homeostasis in adults, via phosphorylation of PKP1 and subsequent promotion of keratinocyte differentiation and cell adhesion. It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.
Cellular Location	Cytoplasm {ECO:0000256\|ARBA:ARBA00004496}. Membrane {ECO:0000256\|ARBA:ARBA00004170}; Peripheral membrane protein {ECO:0000256\|ARBA:ARBA00004170}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.