BCAT2

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using BCAT2 mAb against human BCAT2 (AA: 259-393) recombinant protein. (Expected MW is 41.5 kDa)
Figure 3:Western blot analysis using BCAT2 mAb against HEK293 (1) and BCAT2 (AA: 259-393)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of HeLa cells using BCAT2 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O15382
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G3A11
Isotype	Mouse IgG1
Calculated MW	44288 Da
Immunogen	Purified recombinant fragment of human BCAT2 (AA: 259-393) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	587
Other Names	BCAM; BCT2; PP18; BCATM
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BCAT2 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BCAT2 {ECO:0000303\|PubMed:31177572, ECO:0000312\|HGNC:HGNC:977}
Function	Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:17050531, PubMed:25653144, PubMed:8702755). Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes (By similarity). Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning (By similarity). May also function as a transporter of branched chain alpha-keto acids (By similarity).
Cellular Location	Mitochondrion {ECO:0000250\|UniProtKB:O35854}.
Tissue Location	Ubiquitous..

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.