BCAT2

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using BCAT2 mAb against human BCAT2 (AA: 259-393) recombinant protein. (Expected MW is 41.5 kDa)
Figure 3:Western blot analysis using BCAT2 mAb against HEK293 (1) and BCAT2 (AA: 259-393)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of HeLa cells using BCAT2 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O15382
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G3A11
Isotype	Mouse IgG1
Calculated MW	44288 Da
Immunogen	Purified recombinant fragment of human BCAT2 (AA: 259-393) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	587
Other Names	BCAM; BCT2; PP18; BCATM
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BCAT2 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BCAT2 {ECO:0000303\|PubMed:31177572, ECO:0000312\|HGNC:HGNC:977}
Function	Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:17050531, PubMed:25653144, PubMed:8702755). Branched chain amino acid catabolism plays a role in adipocyte differentiation by providing lipogenic acetyl-CoA pools in differentiated adipocytes (By similarity). Mechanistically, acetyl-CoA derived from branched chain amino acid catabolism is used by EP300/p300 to acetylate and inhibit PRDM16, thereby preventing adipose tissue browning (By similarity). May also function as a transporter of branched chain alpha-keto acids (By similarity).
Cellular Location	Mitochondrion {ECO:0000250\|UniProtKB:O35854}.
Tissue Location	Ubiquitous..

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.