TFAP2B

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using TFAP2B mAb against human TFAP2B (AA: 84-193) recombinant protein. (Expected MW is 38 kDa)
Figure 3:Western blot analysis using TFAP2B mAb against HEK293 (1) and TFAP2B (AA: 84-193)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using TFAP2B mouse mAb against SK-N-SH (1) cell lysate.
Figure 5:Flow cytometric analysis of SK-N-SH cells using TFAP2B mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q92481
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6F7G1
Isotype	Mouse IgG2b
Calculated MW	50474 Da
Immunogen	Purified recombinant fragment of human TFAP2B (AA: 84-193) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	7021
Other Names	PDA2; AP-2B; AP2-B
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TFAP2B is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TFAP2B
Function	Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:Q61313}. Note=In the brain, localizes to the arcuate hypothalamic nucleus, the ventromedial hypothalamic nucleus and the accumbens nucleus of the ventral striatum. {ECO:0000250\|UniProtKB:Q61313}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.