GRIA2

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GRIA2 mAb against human GRIA2 (AA: 35-175) recombinant protein. (Expected MW is 41.9 kDa)
Figure 3:Western blot analysis using GRIA2 mAb against HEK293 (1) and GRIA2 (AA: 35-175)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of SK-N-SH cells using GRIA2 mouse mAb (green) and negative control (red).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P42262
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7A7D12
Isotype	Mouse IgG2b
Calculated MW	98821 Da
Immunogen	Purified recombinant fragment of human GRIA2 (AA: 35-175) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2891
Other Names	GLUR2; GLURB; GluA2; HBGR2; GluR-K2
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GRIA2 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GRIA2 (HGNC:4572)
Function	Ionotropic glutamate receptor that functions as a ligand- gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:20614889, PubMed:31300657, PubMed:8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed:14687553). Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed:20614889, PubMed:8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Postsynaptic cell membrane; Multi-pass membrane protein. Postsynaptic density membrane {ECO:0000250\|UniProtKB:P23819}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P23819}. Note=Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression (By similarity). Displays a somatodendritic localization and is excluded from axons in neurons (By similarity). {ECO:0000250\|UniProtKB:P19491, ECO:0000250\|UniProtKB:P23819}