GRM5

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)
Figure 3:Western blot analysis using GRM5 mAb against HEK293 (1) and GRM5 (AA: extra 458-580)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using GRM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GRM5 mouse mAb with DAB staining.
1/200 - 1/1000

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P41594
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1A11D10
Isotype	Mouse IgG1
Calculated MW	132469 Da
Immunogen	Purified recombinant fragment of human GRM5 (AA: extra 458-580) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2915
Other Names	mGlu5; GPRC1E; MGLUR5; PPP1R86
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GRM5 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GRM5
Synonyms	GPRC1E, MGLUR5
Function	G protein-coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol- calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Cellular Location	Cell membrane; Multi-pass membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.