GLRB

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using GLRB mAb against human GLRB (AA: extra 23-160) recombinant protein. (Expected MW is 41.8 kDa)
Figure 3:Western blot analysis using GLRB mAb against HEK293 (1) and GLRB (AA: extra 23-160)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using GLRB mouse mAb (green) and negative control (red).
Figure 5:Immunofluorescence analysis of Hela cells using GLRB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P48167
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3B8A8
Isotype	Mouse IgG2b
Calculated MW	56122 Da
Immunogen	Purified recombinant fragment of human GLRB (AA: extra 23-160) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	2743
Other Names	HKPX2
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/200 - 1/1000 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GLRB is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GLRB
Function	Subunit of heteromeric glycine-gated chloride channels (PubMed:11929858, PubMed:15302677, PubMed:16144831, PubMed:22715885, PubMed:23238346, PubMed:25445488, PubMed:34473954, PubMed:8717357). Plays an important role in the down-regulation of neuronal excitability (PubMed:11929858, PubMed:23238346). Contributes to the generation of inhibitory postsynaptic currents (PubMed:25445488).
Cellular Location	Postsynaptic cell membrane {ECO:0000250\|UniProtKB:P48168}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P23415}. Synapse {ECO:0000250\|UniProtKB:P48168} Cell projection, dendrite {ECO:0000250\|UniProtKB:P48168}. Cell membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P23415}. Cytoplasm. Note=Retained in the cytoplasm upon heterologous expression by itself. Coexpression with GPHN promotes expression at the cell membrane (PubMed:12684523). Coexpression with GLRA1, GLRA2 or GLRA3 promotes expression at the cell membrane {ECO:0000250\|UniProtKB:P20781, ECO:0000269\|PubMed:12684523}