ADAP1

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ADAP1 mAb against human ADAP1 (AA: 240-370) recombinant protein. (Expected MW is 41.6 kDa)
Figure 3:Western blot analysis using ADAP1 mAb against HEK293 (1) and ADAP1 (AA: 240-370)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ADAP1 mouse mAb against C6 (1), A549 (2), and HepG2 (3) cell lysate.
Figure 5:Flow cytometric analysis of K562 cells using ADAP1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O75689
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Clone Names	3E2D9
Isotype	Mouse IgG1
Calculated MW	43395 Da
Immunogen	Purified recombinant fragment of human ADAP1 (AA: 240-370) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	11033
Other Names	GCS1L; CENTA1; P42ip4
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ADAP1 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ADAP1
Synonyms	CENTA1
Function	GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Regulates the incorporation of CD63 and CD9 into multivesicular bodies (PubMed:38682696).
Cellular Location	Nucleus. Cytoplasm. Note=Recruited to the plasma membrane upon epidermal growth factor-dependent activation of phosphatidylinositol 4,5-diphosphate (PtdInsP2) 3-kinase
Tissue Location	Expressed at highest levels in brain and at lower levels in peripheral blood leukocytes.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.