AQP2

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using AQP2 mAb against human AQP2 (AA: 149-271) recombinant protein. (Expected MW is 39.4 kDa)
Figure 3:Western blot analysis using AQP2 mAb against HEK293 (1) and AQP2 (AA: 149-271)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using AQP2 mouse mAb against K562 (1), HeLa (2), HCT116 (3), and SW480 (4) cell lysate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P41181
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7H8B5
Isotype	Mouse IgG1
Calculated MW	28837 Da
Immunogen	Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	359
Other Names	AQP-CD; WCH-CD
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	AQP2 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	AQP2 (HGNC:634)
Function	Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:15509592, PubMed:7510718, PubMed:7524315, PubMed:8140421, PubMed:8584435). Plays an essential role in renal water homeostasis (PubMed:15509592, PubMed:7524315, PubMed:8140421). Could also be permeable to glycerol (PubMed:8584435).
Cellular Location	Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250\|UniProtKB:P34080}; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane (PubMed:15509592). Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane (PubMed:15509592). PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated (By similarity) {ECO:0000250\|UniProtKB:P34080, ECO:0000269\|PubMed:15509592}
Tissue Location	Expressed in collecting tubules in kidney medulla (at protein level) (PubMed:7510718). Detected in kidney (PubMed:7510718).