CD147

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD147 mAb against human CD147 (AA: extra 138-323) recombinant protein. (Expected MW is 46 kDa)
Figure 3:Western blot analysis using CD147 mAb against HEK293 (1) and CD147 (AA: extra 138-323)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of K562 cells using CD147 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD147 mouse mAb with DAB staining.
1/200 - 1/1000

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P35613
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7H4D4
Isotype	Mouse IgG1
Calculated MW	42200 Da
Immunogen	Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	682
Other Names	BSG; OK; 5F7; TCSF; EMMPRIN
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD147 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BSG (HGNC:1116)
Function	[Isoform 1]: Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
Cellular Location	Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [Isoform 2]: Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P26453}. Endosome Endoplasmic reticulum membrane; Single- pass type I membrane protein {ECO:0000250\|UniProtKB:P26453} Basolateral cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P26453} [Isoform 4]: Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P26453}
Tissue Location	[Isoform 1]: Retina-specific (PubMed:25957687). Expressed in retinal cone photoreceptors (at protein level) (PubMed:25957687). [Isoform 3]: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus.