CD147

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD147 mAb against human CD147 (AA: extra 138-323) recombinant protein. (Expected MW is 46 kDa)
Figure 3:Western blot analysis using CD147 mAb against HEK293 (1) and CD147 (AA: extra 138-323)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of K562 cells using CD147 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD147 mouse mAb with DAB staining.
1/200 - 1/1000

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P35613
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7H4D4
Isotype	Mouse IgG1
Calculated MW	42200 Da
Immunogen	Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	682
Other Names	BSG; OK; 5F7; TCSF; EMMPRIN
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD147 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BSG (HGNC:1116)
Function	[Isoform 1]: Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
Cellular Location	Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [Isoform 2]: Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P26453}. Endosome Endoplasmic reticulum membrane; Single- pass type I membrane protein {ECO:0000250\|UniProtKB:P26453} Basolateral cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P26453} [Isoform 4]: Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P26453}
Tissue Location	[Isoform 1]: Retina-specific (PubMed:25957687). Expressed in retinal cone photoreceptors (at protein level) (PubMed:25957687). [Isoform 3]: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus.