CD195

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD195 mAb against human CD195 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
Figure 3:Western blot analysis using CD195 mAb against HEK293 (1) and CD195 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CD195 mouse mAb against L-02 (1) cell lysate.
Figure 5:Flow cytometric analysis of HL-60 cells using CD195 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P51681
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	6H3D5
Isotype	Mouse IgG1
Calculated MW	40524 Da
Immunogen	Purified recombinant fragment of human CD195 (AA: extra mix) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	1234
Other Names	CCR5; CKR5; CCR-5; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD195 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCR5 (HGNC:1606)
Synonyms	CMKBR5
Function	Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed:30713770).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.