CD195

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD195 mAb against human CD195 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
Figure 3:Western blot analysis using CD195 mAb against HEK293 (1) and CD195 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CD195 mouse mAb against MOLT4 (1), L-02 (2), SPA-C-1 (3), A549 (4), and C6 (5) cell lysate.
Figure 5:Flow cytometric analysis of K562 cells using CD195 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD195 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD195 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P51681
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Clone Names	6G11D1
Isotype	Mouse IgG1
Calculated MW	40524 Da
Immunogen	Purified recombinant fragment of human CD195 (AA: extra mix) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	1234
Other Names	CCR5; CKR5; CCR-5; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD195 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CCR5 (HGNC:1606)
Synonyms	CMKBR5
Function	Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed:30713770).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.