P2RY4

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using P2RY4 mAb against human P2RY4 (AA: extra mix) recombinant protein. (Expected MW is 10.1 kDa)
Figure 3:Western blot analysis using P2RY4 mAb against HEK293 (1) and P2RY4 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using P2RY4 mouse mAb (green) and negative control (red).
Figure 4:Immunofluorescence analysis of Hela cells using P2RY4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using P2RY4 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using P2RY4 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P51582
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3B4H6
Isotype	Mouse IgG1
Calculated MW	40963 Da
Immunogen	Purified recombinant fragment of human P2RY4 (AA: extra mix) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	5030
Other Names	NRU; P2P; UNR; P2Y4
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~ 1/100 - 1/500 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	P2RY4 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	P2RY4
Synonyms	NRU
Function	Receptor for UTP and UDP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Not activated by ATP or ADP.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Pancreas.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.