CD193

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD193 mAb against human CD193 (AA: extra mix) recombinant protein. (Expected MW is 37.2 kDa)
Figure 3:Western blot analysis using CD193 mAb against HEK293 (1) and CD193 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of HL-60 cells using CD193 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of K562 cells using CD193 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P51677
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8E9H10
Isotype	Mouse IgG1
Calculated MW	41044 Da
Immunogen	Purified recombinant fragment of human CD193 (AA: extra mix) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	1232
Other Names	CKR3; CCR3; CMKBR3; CC-CKR-3
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD193 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCR3
Synonyms	CMKBR3
Function	G protein-coupled receptor (GPCR) that plays a key role in the immune system by regulating the migration and activation of white blood cells in response to chemokines (PubMed:28994588). Selectively interacts with eosinophil-attracting chemokines such as eotaxin/CCL11, eotaxin-2/CCL24 and eotaxin-3/CCL26 (PubMed:7622448, PubMed:8642344, PubMed:8676064). Ligand binding triggers intracellular signaling that leads to chemotaxis of immune cells. Mechanistically, signals through GNA14 or GNA16 to induce stimulation of phospholipase Cbeta/PLCB2 and subsequently chemotaxis (PubMed:18406577). Alternatively, transduces signal via GNAI1 resulting in elevated intracellular calcium levels and activation of the PI3K/AKT pathway (PubMed:8676064, PubMed:35570218). May also act as a possible functional receptor for NARS1 (PubMed:30171954).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	In eosinophils as well as trace amounts in neutrophils and monocytes.