CD10

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD10 mAb against human CD10 (AA: extra 549-750) recombinant protein. (Expected MW is 48.8 kDa)
Figure 3:Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: extra 549-750)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of HL-60 cells using CD10 mouse mAb (green) and negative control (red).
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD10 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P08473
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	3G6B11
Isotype	Mouse IgG1
Calculated MW	85514 Da
Immunogen	Purified recombinant fragment of human CD10 (AA: extra 549-750) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	4311
Other Names	NEP; SFE; MME; CALLA; CMT2T; SCA43
Dilution	WB~~ 1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD10 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MME {ECO:0000303\|PubMed:27588448, ECO:0000312\|HGNC:HGNC:7154}
Function	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
Cellular Location	Cell membrane; Single-pass type II membrane protein