CD104

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD104 mAb against human CD104 (AA: extra 29-206) recombinant protein. (Expected MW is 46.5 kDa)
Figure 3:Western blot analysis using CD104 mAb against HEK293 (1) and CD104 (AA: extra 29-206)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CD104 mouse mAb against A549 (1), A431 (2), and SW620 (3) cell lysate.
Figure 5:Flow cytometric analysis of HL-60 cells using CD104 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD104 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P16144
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	5G3G5
Isotype	Mouse IgG2a
Calculated MW	202167 Da
Immunogen	Purified recombinant fragment of human CD104 (AA: extra 29-206) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	3691
Other Names	ITGB4; GP150
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD104 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ITGB4
Function	Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes
Tissue Location	Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.