CD158E1

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD158E1 mAb against human CD158E1 (AA: extra 206-340) recombinant protein. (Expected MW is 40.5 kDa)
Figure 3:Western blot analysis using CD158E1 mAb against HEK293 (1) and CD158E1 (AA: extra 206-340)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of HL-60 cells using CD158E1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Ramos cells using CD158E1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P43629
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2C3A10
Isotype	Mouse IgG1
Calculated MW	49098 Da
Immunogen	Purified recombinant fragment of human CD158E1 (AA: extra 206-340) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	3811
Other Names	KIR3DL1; KIR; NKB1; NKAT3; NKB1B; NKAT-3; KIR3DL1/S1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD158E1 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KIR3DL1 (HGNC:6338)
Synonyms	CD158E, NKAT3, NKB1
Function	Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.
Cellular Location	Cell membrane; Single-pass type I membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.