CD112

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD112 mAb against human CD112 (AA: extra 58-212) recombinant protein. (Expected MW is 45.4 kDa)
Figure 3:Western blot analysis using CD112 mAb against HEK293 (1) and CD112 (AA: extra 58-212)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Ramos cells using CD112 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q92692
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	4A11B12
Isotype	Mouse IgG1
Calculated MW	57742 Da
Immunogen	Purified recombinant fragment of human CD112 (AA: extra 58-212) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	5819
Other Names	NECTIN2; HVEB; PRR2; PVRL2; PVRR2
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD112 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NECTIN2 (HGNC:9707)
Synonyms	HVEB, PRR2, PVRL2
Function	Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705). Probable cell adhesion protein (PubMed:9657005).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Ubiquitous.