B7H3

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using B7H3 mAb against human B7H3 (AA: extra 29-466) recombinant protein. (Expected MW is 76.9 kDa)
Figure 4:Flow cytometric analysis of Jurkat cells using B7H3 mouse mAb (green) and negative control (red).
Figure 3:Immunofluorescence analysis of Hela cells using B7H3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using B7H3 mouse mAb with DAB staining.
1/200 - 1/1000

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q5ZPR3
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	8C1C2
Isotype	Mouse IgG1
Calculated MW	57235 Da
Immunogen	Purified recombinant fragment of human B7H3 (AA: extra 29-466) expressed in HEK293 cells.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	80381
Other Names	CD276; B7-H3; B7RP-2; 4Ig-B7-H3
Dilution	WB~~ 1/500 - 1/2000 IHC~~ 1/200 - 1/1000 ICC~~ 1/100 - 1/500 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	B7H3 is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CD276
Synonyms	B7H3
Function	May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Cellular Location	Membrane; Single-pass type I membrane protein
Tissue Location	Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.