APAF1

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)
Figure 3:Western blot analysis using APAF1 mAb against HEK293 (1) and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using APAF1 mouse mAb (green) and negative control (red).
Figure 4:Immunofluorescence analysis of Hela cells using APAF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	O14727
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2H9A1
Isotype	Mouse IgG2b
Calculated MW	141840 Da
Immunogen	Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	317
Other Names	CED4; APAF-1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~ 1/100 - 1/500 E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	APAF1 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	APAF1 (HGNC:576)
Synonyms	KIAA0413
Function	Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Cellular Location	Cytoplasm.
Tissue Location	Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.