ACTR3

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ACTR3 mAb against human ACTR3 (AA: 287-418) recombinant protein. (Expected MW is 41.1 kDa)
Figure 3:Western blot analysis using ACTR3 mAb against HEK293 (1) and ACTR3 (AA: 287-418)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 5:Western blot analysis using ACTR3 mouse mAb against NIH/3T3 (1), A549 (2), and CHO3D10 (2) cell lysate.
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ACTR3 mouse mAb with DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P61158
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Clone Names	3G2G1
Isotype	Mouse IgG1
Calculated MW	47371 Da
Immunogen	Purified recombinant fragment of human ACTR3 (AA: 287-418) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	10096
Other Names	ARP3
Dilution	WB~~ 1/500 - 1/2000 IHC~~1/200 - 1/1000 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ACTR3 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACTR3
Synonyms	ARP3
Function	ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9000076). Seems to contact the pointed end of the daughter actin filament (PubMed:9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed:29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:17220302, PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947). Plays a role in ciliogenesis (PubMed:20393563).
Cellular Location	Cytoplasm, cytoskeleton. Cell projection. Nucleus. Note=In pre- apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554)