CD16

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD16 mAb against human CD16 (AA: extra 17-208) recombinant protein. (Expected MW is 47.8 kDa)
Figure 3:Western blot analysis using CD16 mAb against HEK293 (1) and CD16 (AA: extra 17-208)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Ramos cells using CD16 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	P08637
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	2G10A9
Isotype	Mouse IgG1
Calculated MW	29089 Da
Immunogen	Purified recombinant fragment of human CD16 (AA: extra 17-208) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	FCGR3A; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CD16 is for research use only and not for use in diagnostic or therapeutic procedures.