PD1

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Figure 3:Western blot analysis using PD1 mouse mAb against MOLT4 (1), K562 (2), Ramos (3), HL-60 (4), THP-1 (5), and U937 (6) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using PD1 mouse mAb (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, ICC, E
Primary Accession	Q15116
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	1D6F6
Isotype	Mouse IgG1
Calculated MW	31647 Da
Immunogen	Purified recombinant fragment of human PD1 (AA: extra 21-170) expressed in HEK293 cells.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Gene ID	5133
Other Names	PDCD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Dilution	WB~~ 1/500 - 1/2000 IHC~~1:100~500 ICC~~N/A E~~ 1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PD1 is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PDCD1 {ECO:0000303\|PubMed:7851902, ECO:0000312\|HGNC:HGNC:8760}
Function	Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005, PubMed:31754127, PubMed:32184441, PubMed:37208329). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005, PubMed:26602187). Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation (PubMed:32184441). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:32184441).
Cellular Location	Cell membrane; Single-pass type I membrane protein