DNAJA1 Recombinant Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP
Primary Accession	P31689
Other Accession	P31689
Host	Rabbit
Clonality	Recombinant
Calculated MW	44868 Da
Physical State	Liquid
Immunogen	A synthesized peptide derived from human DNAJA1
Epitope Specificity	350-397/397
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
SIMILARITY	Contains 1 CR-type zinc finger. Contains 1 J domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.

Additional Information
Gene ID	3301
Other Names	DJ-2; DjA1; HDJ2; HSDJ; HSJ-2; HSJ2; HSPF4; NEDD7; hDJ-2; DNJA1_HUMAN; DNAJA1; DnaJ protein homolog 2; Heat shock 40 kDa protein 4; Heat shock protein J2 (HSJ-2); Human DnaJ protein 2 (hDj-2); DNAJ2; DNJA1_MOUSE; DNJA1_RAT; DnaJ-like protein 1; Rdj1; DnaJ heat shock protein family (Hsp40) member A1; DnaJ (Hsp40) homolog, subfamily A, member 1; neural precursor cell expressed, developmentally down-regulated 7
Dilution	WB=1:500-2000,IP=1:20-50,Flow-Cyt=1:50-100,ICC/IF=1:50-200
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DNAJA1
Synonyms	DNAJ2, HDJ2, HSJ2, HSPF4
Function	Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co- chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).
Cellular Location	Membrane; Lipid- anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm, perinuclear region. Mitochondrion Note=Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.
Tissue Location	Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.