癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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EHHADH Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q08426 |
| Other Accession | Q08426 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 79495 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human EHHADH |
| Epitope Specificity | 631-723/723 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. |
| SUBUNIT | Monomer. |
| Post-translational modifications | Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | EHHADH is one of three preoxisomal enzymes of the classic peroxisomal Beta-oxidation cycle involved in the catabolism of free fatty acids.Most dietary long chain fatty acids are oxidized in the mitochondria, but very-long-chain fatty acids (VLCFAs) must be shortened first by peroxisomal Beta-oxidation prior to complete oxidation in mitochondria. |
| Gene ID | 1962 |
|---|---|
| Other Names | ECHD; FRTS3; L-PBE; LBFP; LBP; MFE1; PBFE; 1300002P22Rik; HD; MFP; MFP-1; MFP1; ECHP_HUMAN; EHHADH; PBE; L-bifunctional protein (LBP); Multifunctional enzyme 1 (MFE1); ECHP_MOUSE; L-peroxisomal bifunctional enzyme (L-PBE); Multifunctional protein 1 (MFP-1); enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | EHHADH (HGNC:3247) |
|---|---|
| Synonyms | ECHD |
| Function | Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl- CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl- 2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3- hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta- oxidation (PubMed:15060085). |
| Cellular Location | Peroxisome. |
| Tissue Location | Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP100119
