CHSY1 Rabbit pAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	Q86X52
Other Accession	Q86X52
Host	Rabbit
Clonality	Polyclonal
Calculated MW	91784 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human CHSY1
Epitope Specificity	51-150/802
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a member of the chondroitin N-acetylgalactosaminyltransferase family. These enzymes possess dual glucuronyltransferase and galactosaminyltransferase activity and play critical roles in the biosynthesis of chondroitin sulfate, a glycosaminoglycan involved in many biological processes including cell proliferation and morphogenesis. Decreased expression of this gene may play a role in colorectal cancer, and mutations in this gene are a cause of temtamy preaxial brachydactyly syndrome. [provided by RefSeq, Dec 2011]

Additional Information
Gene ID	22856
Other Names	CHSY; CSS1; ChSy-1; TPBS; skt; CHSS1_HUMAN; CHSY1; Chondroitin glucuronyltransferase 1; Chondroitin synthase 1 (ChSy-1); Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1; N-acetylgalactosaminyltransferase 1; 2.4.1.175; 2.4.1.226; KIAA0990; CHSS1_MOUSE; chondroitin sulfate synthase 1; carbohydrate (chondroitin) synthase 1
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CHSY1 (HGNC:17198)
Synonyms	CHSY, CSS1, KIAA0990
Function	Catalytic component of CHSY1-CHPF2 and CHSY1-CHPF chondroitin sulfate synthase complexes (PubMed:41298522). Has both beta-1,3- glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N- acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer (PubMed:11514575, PubMed:12716890). Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling.
Cellular Location	Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted
Tissue Location	Ubiquitous, with the highest levels in placenta. Detected at low levels in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, adrenal gland, mammary gland, stomach, small intestine, lung and peripheral blood leukocytes