CRCT1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

CRCT1 Antibody (N-term) (Cat. #AP10028a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CRCT1 antibody detected the CRCT1 protein (arrow).
CRCT1 antibody (N-term) (Cat. #AP10028a) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRCT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CRCT1 Antibody (N-term) (Cat. #AP10028a) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q9UGL9
Other Accession	NP_061933.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	9736 Da
Antigen Region	1-30 aa

Additional Information
Gene ID	54544
Other Names	Cysteine-rich C-terminal protein 1, Protein NICE-1, CRCT1, C1orf42, NICE1
Target/Specificity	This CRCT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CRCT1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CRCT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.