癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Cdc25B Recombinant Rabbit mAb
- 产品详情
- 实验流程
Application 
| WB, IP |
|---|---|
| Primary Accession | P30305 |
| Other Accession | P30305 |
| Host | Rabbit |
| Clonality | Recombinant |
| Calculated MW | 64987 Da |
| Physical State | Liquid |
| Immunogen | A synthesized peptide derived from human CDC25B |
| Epitope Specificity | 175-250 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the MPI phosphatase family.Contains 1 rhodanese domain. |
| SUBUNIT | Interacts with MAPK14 and 14-3-3 proteins. |
| Post-translational modifications | Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. |
| Gene ID | 994 |
|---|---|
| Other Names | MPIP2; MPIP2_HUMAN; CDC25B; Dual specificity phosphatase Cdc25B; 3.1.3.48; CDC25HU2; MPIP2_MOUSE; Cdc25m2; MPIP2_RAT; cell division cycle 25B; CDC25 homolog B (S. pombe); M-phase inducer phosphatase 2 |
| Dilution | WB=1:500-2000,IP=1:20-50,Flow-Cyt=1:50-100 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | CDC25B |
|---|---|
| Synonyms | CDC25HU2 |
| Function | Tyrosine protein phosphatase which functions as a dosage- dependent inducer of mitotic progression (PubMed:1836978, PubMed:20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed:20360007). Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner (PubMed:17332740). The three isoforms seem to have a different level of activity (PubMed:1836978). |
| Cellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP100313
