HA Tag Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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- 实验流程
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Application ![]()
| WB, IF, CHIP, E |
---|---|
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Other Names | Glutathione S-transferase |
---|---|
Target/Specificity | KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen. |
Dilution | WB~~1:1000 IF~~1:10~50 CHIP~~1:100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |

Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
REFERENCES
Marco. et.al., J Biol Chem. (2009) February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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