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HA Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HA Tag Antibody AP1012A
    Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody (Cat. #AP1012A). AP1012A was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - HA Tag Antibody AP1012A
    Western blot analysis of anti-HA tag pab(Cat. #AP1012a)in HA-tagged recombinant protein bacterial lysate.
  • 3 - HA Tag Antibody AP1012A
    GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
  • 9 - HA Tag Antibody AP1012A
    ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, CHIP, E
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Other Names Glutathione S-transferase
Target/Specificity KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Dilution WB~~1:1000
IF~~1:10~50
CHIP~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.

REFERENCES

Marco. et.al., J Biol Chem. (2009) February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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