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FLAG tag antibody (DYKDDDDK)

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-FLAG tag antibody (DYKDDDDK) at 1:2000 dilution Lane 1: 12-tag whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-FLAG tag antibody (DYKDDDDK) at 1:2000 dilution Lane 1: 12-tag whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-FLAG tag antibody (DYKDDDDK) at 1:2000 dilution Lane 1: 12-tag recombinant protein Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-FLAG tag antibody (DYKDDDDK) at 1:2000 dilution Lane 1: 12-tag whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Tag-FLAG. 2x at 1:2000 dilution + 12-tag lysate Lysates/proteins at 20ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysate from 12tag protein, using FLAG tag antibody (DYKDDDDK)(Cat. #AP1013a). AP1013a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot analysis of lysate from 12tag recombinant protein, using FLAG tag antibody (DYKDDDDK)(Cat. #AP1013a). AP1013a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot analysis of lysate from 12tag recombiant protein, using Tag-FLAG. 2x Antibody(Cat. #AP1013a). AP1013a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot analysis of lysate from 12tag recombinant protein , using FLAG tag antibody (DYKDDDDK)(Cat. #AP1013a). AP1013a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG

Additional Information
Other Names	Tag from influenza hemagglutinin protein
Target/Specificity	KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	FLAG tag antibody (DYKDDDDK) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).

REFERENCES

Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 1996;20(1):136-141

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¥ 1,250.00

Cat# AP1013A

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

询价

FLAG tag antibody (DYKDDDDK)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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