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JMJD3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - JMJD3 Antibody (N-term) AP1022A
    Western blot analysis of anti-JMJD3 (N-term) Pab (AP1022a) in Hela cell line lysates. JMJD3 (arrow) was detected using the purified Pab.
  • 14 - JMJD3 Antibody (N-term) AP1022A
    Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 (N-term) (Cat.#AP1022a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - JMJD3 Antibody (N-term) AP1022A
    Confocal immunofluorescent analysis of JMJD3 Antibody (N-term) (Cat#AP1022a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession O15054
Other Accession Q5NCY0
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 176632 Da
Additional Information
Gene ID 23135
Other Names Lysine-specific demethylase 6B, 11411-, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, KDM6B, JMJD3, KIAA0346
Target/Specificity This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJMJD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM6B
Synonyms JMJD3, KIAA0346
Function Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914). Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed:17825402, PubMed:17851529, PubMed:17713478, PubMed:18003914). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4- containing SWI/SNF remodeling complex (By similarity).
Cellular Location Nucleus.
Research Areas

BACKGROUND

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

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