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>   首页   >   产品   >   一抗   >   细胞生物学   >   RPS6 (Ser240/244) Antibody   

RPS6 (Ser240/244) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPS6 (Ser240/244) Antibody AP10275a
    Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244)(Cat. #AP10275a). AP10275a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • 14 - RPS6 (Ser240/244) Antibody AP10275a
    RPS6 antibody (Ser240/244) (Cat. #AP10275a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - RPS6 (Ser240/244) Antibody AP10275a
    RPS6 Antibody (Ser240/244) (Cat. #AP10275a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P62753
Other Accession P39017, P62755, P62754, Q4R4K6, P47838, Q5E995, NP_001001.2
Reactivity Human
Predicted Bovine, Chicken, Monkey, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 28681 Da
Antigen Region 220-249 aa
Additional Information
Gene ID 6194
Other Names 40S ribosomal protein S6, Phosphoprotein NP33, RPS6
Target/Specificity This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
Dilution IHC-P~~1:100~500
FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS6 (Ser240/244) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6 {ECO:0000303|PubMed:29563586, ECO:0000312|HGNC:HGNC:10429}
Function Component of the 40S small ribosomal subunit (PubMed:23636399, PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Cellular Location Cytoplasm. Nucleus, nucleolus
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

REFERENCES

Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008)
Robledo, S., et al. RNA 14(9):1918-1929(2008)
Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008) :
Ma, X.M., et al. Cell 133(2):303-313(2008)

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