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WFDC12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

WFDC12 Antibody (C-term) (Cat. #AP10369b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WFDC12 antibody detected the WFDC12 protein (arrow).
WFDC12 antibody (C-term) (Cat. #AP10369b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WFDC12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
WFDC12 Antibody (C-term) (Cat. #AP10369b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q8WWY7
Other Accession	NP_543145.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Other Names	WAP four-disulfide core domain protein 12, Putative protease inhibitor WAP12, Whey acidic protein 2, WFDC12, C20orf122, WAP2
Target/Specificity	This WFDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the C-terminal region of human WFDC12.
Dilution	WB~~1:1000 IHC-P~~1:100~1:500 FC~~1:100~1:500
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	WFDC12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	WFDC12
Synonyms	C20orf122, WAP2
Function	Antibacterial protein. Putative acid-stable proteinase inhibitor.
Cellular Location	Secreted.
Tissue Location	Highly expressed in prostate, skin, lung and esophagus. Weakly expressed in skeletal muscle, epididymis, kidney, trachea, salivary gland, testis and seminal vesicle EMBL; AY037803; AAK68848.1; -; mRNA EMBL; AY358678; AAQ89041.1; -; mRNA EMBL; Z93016; -; NOT_ANNOTATED_CDS; Genomic_DNA CCDS; CCDS13343.1; - RefSeq; NP_543145.1; NM_080869.1 UniGene; Hs.352180; - ProteinModelPortal; Q8WWY7; - SMR; Q8WWY7; - BioGrid; 126126; 1 IntAct; Q8WWY7; 6 STRING; 9606.ENSP00000361871; - MEROPS; I17.003; - iPTMnet; Q8WWY7; - PhosphoSitePlus; Q8WWY7; - BioMuta; WFDC12; - DMDM; 24212610; - PaxDb; Q8WWY7; - PeptideAtlas; Q8WWY7; - PRIDE; Q8WWY7; - ProteomicsDB; 74959; - DNASU; 128488; - Ensembl; ENST00000372785; ENSP00000361871; ENSG00000168703 GeneID; 128488; - KEGG; hsa:128488; - UCSC; uc002xnf.2; human CTD; 128488; - EuPathDB; HostDB:ENSG00000168703.5; - GeneCards; WFDC12; - HGNC; HGNC:16115; WFDC12 MIM; 609872; gene neXtProt; NX_Q8WWY7; - OpenTargets; ENSG00000168703; - PharmGKB; PA25663; - eggNOG; ENOG410JHHC; Eukaryota eggNOG; ENOG41119G0; LUCA GeneTree; ENSGT00390000012286; - HOGENOM; HOG000133110; - HOVERGEN; HBG055877; - InParanoid; Q8WWY7; - OMA; WEAKSPG; - OrthoDB; 1560605at2759; - PhylomeDB; Q8WWY7; - TreeFam; TF338375; - GenomeRNAi; 128488; - PRO; PR:Q8WWY7; - Proteomes; UP000005640; Chromosome 20 Bgee; ENSG00000168703; Expressed in 93 organ(s), highest expression level in penis Genevisible; Q8WWY7; HS GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; NAS:UniProtKB GO; GO:0042742; P:defense response to bacterium; ISS:UniProtKB Gene3D; 4.10.75.10; -; 1 InterPro; IPR036645; Elafin-like_sf InterPro; IPR008197; WAP_dom Pfam; PF00095; WAP; 1 PRINTS; PR00003; 4DISULPHCORE SMART; SM00217; WAP; 1 SUPFAM; SSF57256; SSF57256; 1 PROSITE; PS51390; WAP; 1 1: Evidence at protein level; Antibiotic; Antimicrobial; Complete proteome; Disulfide bond; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal SIGNAL 1 23 CHAIN 24 111 WAP four-disulfide core domain protein 12 /FTId=PRO_0000041390 DOMAIN 27 74 WAP. {ECO:0000255\|PROSITE- ProRule:PRU00722} DISULFID 34 62 {ECO:0000255\|PROSITE-ProRule:PRU00722} DISULFID 41 66 {ECO:0000255\|PROSITE-ProRule:PRU00722} DISULFID 49 61 {ECO:0000255\|PROSITE-ProRule:PRU00722} DISULFID 55 70 {ECO:0000255\|PROSITE-ProRule:PRU00722} SEQUENCE 111 AA; 12050 MW; 2DFB39043F1A0997 CRC64; MGSSSFLVLM VSLVLVTLVA VEGVKEGIEK AGVCPADNVR CFKSDPPQCH TDQDCLGERK CCYLHCGFKC VIPVKELEEG GNKDEDVSRP YPEPGWEAKC PGSSSTRCPQ K

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster.

REFERENCES

Clauss, A., et al. Biochem. Biophys. Res. Commun. 333(2):383-389(2005)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Clauss, A., et al. Biochem. J. 368 (PT 1), 233-242 (2002) :
Lundwall, A., et al. Biochem. Biophys. Res. Commun. 290(1):452-456(2002)
Deloukas, P., et al. Nature 414(6866):865-871(2001)

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Cat# AP10369b

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WFDC12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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