癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DENND1B Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q6P3S1 |
| Other Accession | Q3U1T9, NP_001182144.1, NP_001182145.1 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 86552 Da |
| Antigen Region | 39-68 aa |
| Gene ID | 163486 |
|---|---|
| Other Names | DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B |
| Target/Specificity | This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DENND1B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DENND1B (HGNC:28404) |
|---|---|
| Function | Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells (PubMed:20154091, PubMed:20937701, PubMed:24520163, PubMed:26774822). Acts by promoting the exchange of GDP to GTP, converting inactive GDP- bound RAB35 into its active GTP-bound form (PubMed:20154091, PubMed:20937701). Plays a role in clathrin-mediated endocytosis (PubMed:20154091). Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity (PubMed:26774822). Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells (PubMed:26774822). |
| Cellular Location | Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle |
| Tissue Location | Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).
REFERENCES
Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637(2010)
Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)
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