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M4A4A Antibody (N-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - M4A4A Antibody  (N-term) AP10816A
    All lanes : Anti-M4A4A Antibody (N-term) at 1:500 dilution Lane 1: MDA-MB-453 whole cell lysate Lane 2: WiDr whole cell lysate Lane 3: Daudi whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 17kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - M4A4A Antibody  (N-term) AP10816A
    M4A4A Antibody (N-term) (Cat. #AP10816a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the M4A4A antibody detected the M4A4A protein (arrow).
  • 4 - M4A4A Antibody  (N-term) AP10816A
    M4A4A Antibody (N-term) (Cat. #AP10816a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q96JQ5
Other Accession NP_076926.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25441 Da
Antigen Region 37-64 aa
Additional Information
Gene ID 51338
Other Names Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1, MS4A4A, 4SPAN1, CD20L1, MS4A4
Target/Specificity This M4A4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-64 amino acids from the N-terminal region of human M4A4A.
Dilution WB~~1:1000
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM4A4A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name MS4A4A
Synonyms 4SPAN1, CD20L1, MS4A4
Function May be involved in signal transduction as a component of a multimeric receptor complex.
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Variable expression in multiple hemopoietic cell lines
Research Areas

BACKGROUND

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. Alternative splicing of this gene results in several transcript variants; however, not all transcripts have been fully described.

REFERENCES

Liang, Y., et al. Immunogenetics 53(5):357-368(2001)
Liang, Y., et al. Genomics 72(2):119-127(2001)
Ishibashi, K., et al. Gene 264(1):87-93(2001)

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